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KMID : 0358219940210030285
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Korean Journal of Fertility and Sterility
1994 Volume.21 No. 3 p.285 ~ p.296
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The Effect of Ca2+ and Its Specific Time in the Maturation of Mammalian Oocyte
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Abstract
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Follicular oocytes were released from the graafian follicles of ovaries from 3-4 weeks old mice. The spontaenous maturation of these follicular oocytes was inhibited by the treatment of dbcAMP and progesterone and these oocytes were cultured for
2-8hr
in the Modified Hank's balanced salt solution (MHBS).
Ethylenediaminetetraaceticacid (EDTA) and calmoudulin antagonist, trifluoperazine (TFP) were treated to the culture medium in order to investigate whether these chemical agents inhibit calcium uptake into the oocyte and oocyte maturation.
45Ca2+, 10-20μCi/ml was added to the culture medium during the culture period. 45Ca2+ uptake into he oocytes was examined whether and when various kind of oocyte maturation inhibiting agents inhibit or stimulate the influx of calcium into
oocytes.
Dibutyryl cAMP and progesterone decrease 45Ca2+ uptake into the oocytes and synergistic inhibiting effect of dbcAMP and progesterone was prominent at much lower dosages.
Calcium uptake into oocytes seems to be higher during first 2 hour culture period rather than next 4hr culture. After 8hr culture, calcium uptake level of the oocytes which GVBD already took place gradually approached to he level of those which
were
maintained at GV by the treatment of dbcAMP and progesterone.
However, 45Ca2+ uptake into the GV maintained oocytes did not change at all even after 8hr culture period.
In addition, calcium chelating agent, EDTA inhibited calcium uptake into oocytes as well as nuclear maturation of oocytes. Lower dosage used in the present study did not inhibit calcium uptake as well as oocyte maturation.
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KEYWORD
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